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The protein profile of the organelle analyzed by SDS-PAGE revealed a wide range of protein bands, predominantly those corresponding to 85 kDa, a triplet of 67-57 kDa and a doublet of 24-23 kDa. Protease activity in substrate-containing gels, in the presence or absence of protease inhibitors, showed that cysteine proteinase is enriched and very active in the purified fraction.

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Not all kits do a good job of isolating the tiny pieces, he says. Thermo Fisher’s SurePrep™ kits use a special silicon carbide resin to bind the RNA, even micro species. Similarly, Life Technologies’ mir Vana™ kits isolate both small and large RNAs.

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24.07.2019· Trypanosomatid parasites are infectious agents for diseases such as African sleeping sickness, Chagas disease, and leishmaniasis that threaten millions of people, mostly in the emerging world. Trypanosomes compartmentalize glycolytic enzymes to an organelle called the glycosome, a specialized peroxisome. Functionally intact glycosomes are essential for trypanosomatid viability, …

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01.09.2016· 1. Introduction. In July 2011, a group of protesters from Greenpeace, a non-governmental, environmental organization, broke into an experimental farm of the Commonwealth Scientific and Industrial Research Organization (CSIRO), an Australian federal government agency for scientific research, and destroyed the entire crop of genetically modified wheat.

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Obtaining high-quality RNA is the first, and often the most critical, step in performing many molecular techniques such as reverse transcription real-time PCR (RT-qPCR), transcriptome analysis using next-generation sequencing, array analysis, digital PCR, northern analysis, and cDNA library construction.

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Column chromatography is also capable of isolating a nuer of compounds from a mixture. Another advantage of column chromatograph is that very little needs to be known about the compound''s physical properties in order to use this purifiion method, making this technique very valuable when synthesizing or isolating novel compounds, in which little is known about the compound(s).

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