calcium imaging granule cells in thailand

Neuromodulation of Hippocampal Cells and …

Bijak M, Misgeld U (1995) Adrenergic modulation of hilar neuron activity and granule cell inhibition in the guinea-pig hippocampal slice. Neuroscience 67(3):541–550 PubMed CrossRef Google Scholar. Grienberger C, Konnerth A (2012) Imaging calcium in neurons. Neuron 73(5):862–885.

Neuronal Cell Markers - Labome

14.08.2020· Other approaches exists to define neuronal cell types. For example, physiological optical tagging sequencing (PhOTseq) relies on the initial GCaMP calcium imaging labeling of neurons through activation and then the permanent labeling of specific neurons …

SUMOylation Regulates Insulin Exocytosis …

RESEARCH DESIGN AND METHODS We studied islets and β-cells from mice and human donors, as well as INS-1 832/13 cells. Insulin secretion, intracellular Ca 2+, and β-cell exocytosis were monitored after manipulation of the SUMOylation machinery.Granule localization was imaged by total internal reflection fluorescence and electron microscopy; immunoprecipitation and Western blotting were used …

Subcellular Imaging of Voltage and Calcium Signals Reveals

Resource Subcellular Imaging of Voltage and Calcium Signals Reveals Neural Processing In Vivo Helen H. Yang,1,3 Franc¸ois St-Pierre,2,3,4,5 Xulu Sun,1 Xiaozhe Ding,2 Michael Z. Lin,1,2 and Thomas R. Clandinin1,* 1Department of Neurobiology, Stanford University, Stanford, CA 94305, USA 2Department of Bioengineering, Stanford University, Stanford, CA 94305, USA

Bovine Lactadherin as a Calcium-Independent …

Bovine Lactadherin as a Calcium-Independent Imaging Agent of Phosphatidylserine Expressed on the Surface of Apoptotic HeLa Cells J Histochem Cytochem . 2009 Oct;57(10):907-14. doi: 10.1369/jhc.2009.953729.

CellASIC® ONIX Live Cell Analysis | Life Science …

Time-lapse imaging was taken on cells at a 10-minute interval under 400x magnifiion using a FITC and Texas-Red filter. Read the Appliion Note A431 epidermal carcinoma cells ( 85090402 ) were incubated 16 hours overnight with 3µM BioTracker 520 Green Hypoxia Dye ( SCT033 ) in complete DMEM by gravity perfusion at 37°C under normoxic conditions (20% O2) using the CellASIC ® ONIX2 …

Chromogranin A induces the biogenesis of …

Chromogranin A induces the biogenesis of granules with calcium- and actin-dependent dynamics and exocytosis in constitutively secreting cells. Endocrinology. 2012; 153(9):4444-56 (ISSN: 1945-7170)

Imaging and recording subventricular zone progenitor cells

Imaging and recording subventricular zone progenitor cells in live tissue of postnatal mice Benjamin Lacar 1,2‡ , Stephanie Z. Young , Jean-Claude Platel 1,2† and Angélique Bordey 1,2 *

Real-Time Imaging for Ca[sup2]+ Triggered …

Calcium was increased by directly increasing [K+] to depolarize the cells in the prseence of low and high glucose. We found that all insulin granule exocytotic events were directly associated with s in intracellular Ca[sup2]+ concentration.

The role of calcium and cyclic nucleotide …

ABSTRACT In the developing brain, immature neurons migrate from their sites of origin to their final destination, where they reside for the rest of their lives. This active movement of immature neu

Branch-specific Ca2+ influx from Na+ …

Two-photon laser scanning microscopy was used to correlate electrical events detected with whole-cell somatic recordings to Ca 2+ transients in dendrites of olfactory bulb granule cells. A subset of spontaneous subthreshold depolarizing events recorded at the soma were shown to correspond to suprathreshold dendritic, Na-dependent action potentials [APs; dendritic spikes (D-spikes)].

T Cell Calcium Imaging with the Prime 95B - …

Professor Johannes Huppa is using the Prime 95B back-illuminated sCMOS to investigate the activation of T cells using calcium imaging techniques.

Calcium Indiors | Thermo Fisher Scientific - CN

Calcium flux assays are widely used for in-cell measurement of agonist-stimulated and antagonist-inhibited signaling through G protein–coupled receptors (GPCRs), a large and active target class relevant in drug discovery. In neurons, intracellular calcium plays a critical role in induction of synaptic activity and activation of signaling

Calcium exchangeability in subcellular fractions …

The distribution and exchangeability of calcium within subcellular compartments of rat pancreatic islet cells was determined in pulse-chase experiments following isotopic labelling with 45Ca2+. In unstimulated islets the mitochondrial fraction showed the greatest, and the insulin secretory granule fraction the least, uptake of 45Ca2+; subsequently the rate of loss from each compartment during

Stanford researchers accidentally discover a …

Some granule cells lit up as expected, With calcium imaging, the pinpricks of light come from molecules that fluoresce as they trade calcium ions. Often, they light up in green.

Carnegie Mellon study reveals that odor …

Timing is everything. For a mouse trying to discriminate between the scent of a tasty treat and the scent of the neighborhood , timing could mean life or death. In a striking discovery

Cerebellar Craniotomy for In Vivo Calcium …

In vivo, these cell types generate both subcellular calcium transients and trans-glial calcium waves. It is possible to perform in vivo calcium imaging in cerebellar astrocytes. One method involves injection of a repliion-incompetent recoinant adenovirus for gene transfer of a fluorescent calcium …

Calcium signaling resources | Abcam

New cell imaging guide. Calcium assay kits. Neuroscience resources. Apoptosis e-book. Cancer resources. Calcium indiors & ionophores. A collection of articles and protocols highlighting the role calcium plays in different cell types and processes, including …

CALCIUM | meaning in the Caridge English …

calcium definition: 1. a chemical element that is present in teeth, bones, and chalk 2. a chemical element that is…. Learn more.

Stanford scientists find a previously unknown …

20.03.2017· One method, called two-photon calcium imaging, had the resolution Wagner needed to study mouse granule cells in action. In order to study motor control, the team had to …

Live-cell Imaging Techniques | Learn & Share | …

Live-cell imaging provides spatial and temporal information of dynamic events in single cells, cellular networks (in situ) or even whole organisms (in vivo). These features make live-cell imaging a requisite technique for addressing questions in cell biology, cancer research, developmental biology and neuroscience. In recent years, substantial

Interpreting in vivo calcium signals from …

2. Somatic Calcium Signals: as a Proxy for Spiking Activity. Somatic calcium signals have been used to infer spiking activity in neurons. By imaging an ensele of neurons at single-cell resolution, one could delineate task-related neural activity during perceptual or cognitive behavior, 25 map the spatial organization of visually evoked neural responses, 26 and track modifiions to neural

Fura-2 AM (Cell permeant) | Calcium sensitive …

Fura-2 AM (Cell permeant) is a high affinity, cell permeable calcium indior which is ratiometric and UV light excitable. AM ester derivative of Fura-2 . Fura-2 AM (Cell permeant) can noninvasively be loaded into live cells by incubation and is widely used for ratio-imaging microscopy and measuring intracellular calcium elevations in neurons and other excitable cells.

Large-scale imaging of subcellular calcium dynamics of

Large-scale imaging of subcellular calcium dynamics of cortical neurons with G-CaMP6-actin Chiaki Kobayashia, Masamichi Ohkurab, Junichi Nakaib, Norio Matsukia, Yuji Ikegayaa,c and Takuya Sasakia Understanding the information processing performed by

pH-dependent regulation of lysosomal calcium …

Calcium measurements in acidic vacuolar compartments of living cells are few, primarily because calibration of fluorescent probes for calcium requires knowledge of pH and the pH-dependence of the probe calcium-binding affinities. Here we report pH-corrected measurements of free calcium concentrations in lysosomes of mouse macrophages, using both ratiometric and time-resolved …